NEW 600ml Bioreactor Tube

Introducing the new Bioreactor 600! The TPP Bioreactor family has grown from the 50ml tube to now including a 600ml tube. Ideal for larger scale suspensions, parallel experiments, bioreacting, and more, this tube adds versatility, volume, and convenience to any lab.

With a maximum working volume of 400ml and a 0.2um filter cap this tube fits a wide variety of applications. Whether shaking or centrifuging the tube also fits most standard rotors or tube racks for 500ml tubes.

Want to learn more about this product? Contact us at 800-544-9909 or tech@tpp-us.com or watch this video about the Bioreactor Tubes: http://www.youtube.com/watch?v=AdifEb0nezA.

Helpful hints for aseptic techniques

Want better aseptic techniques? For more information click here: http://www.midsci.com/images/Blog/HelpfulHintsBetterAsepticTechnique.pdf.

10cm Tiny Flasks

Have you ever wished you could just grow your adherent cells in a tube, so you could save some time and an extra step in the cell culture hood? TPP 10cm Tiny Flasks solve that problem! The tube is approximately the same size as a 50ml tube, though it has 2 flat sides-one 10cm2 growth area and one flat viewing window for microscopy. The tube will also not fall through a microscope stage like a 35mm dish can. These tubes also have a plastic resting mount to keep the tubes in place in the incubator and to give the tube the optimal angle for the growth surface. The cleverly designed tear-drop shape of the growth surface can be exactly worked with cell scraper as well. See this tube in action at: http://tpp-us.com/docs/tpp-tissue-culture-flask-tube.html.

Using the TPP Easy Read Device

The "Easy Read" PCV (Packed Cell Volume) Tubes provide a quicker, less tedious alternative to counting cells than using a microscope and hemacytometer. Users simply load a sample of cell suspension into the PCV Tube, spin for 1 minute in a microcentrifuge to pellet the cells into the capillary at the bottom of the tube, and then accurately determine the cell pellet volume with the "Easy Read" measuring device. The cell pellet volume can then be utilized in conjunction with pre-determined conversion factors to quickly calculate cell number, protein production, or metabolic activity.

The Easy Read device is a blue metal ruler that measures microliter amounts. The front part of this ruler is flat and allows the user to measure microliter amounts to 0.025ul. Behind the ruler on the front is a metal slant on the back going down toward the higher measurements. The gray metal guide includes both a magnifying glass as well as the tube holder. The bottom of the tube is placed into the holder and rests against the slant on the back of the device. The tube then slides up and down that slant as you move the gray metal piece across the ruler. Once the top of your pellet is flush with the ruler, you can read the measurement through the magnifying glass.

Using the Easy Read device can give you more accurate results over the capillary graduations on the regular PCV tubes. The cost savings in buying the Easy Read device and using the non graduated tubes adds up quickly after multiple samples. http://tpp-us.com/docs/PCV_Easy_Read_Cell_Counting_System.html

Keeping Cells Warm in the TC Hood

Have you ever been in the tissue culture hood and started multi-tasking too much? All of a sudden you have a bunch of tubes holding cells, all of which need to be plated. Or you need to set up a large experiment that takes quite a bit of time, all the while the cells are cooling to room temperature. They have been sitting in the hood for awhile, and really should be warm so that they don't die just waiting to be plated. Thankfully, there is a solution!

The Walkabout Scoop Tray filled with warm (37C) bath beads is the perfect resolution to keeping your samples warm, organized, and alive! The insulated scoop tray can be filled with the warm beads that are already in your water bath, or the beads can be warmed in an incubator. The tray can then be placed in your hood or next to you, as it is only one quarter the size of a standard ice bucket. Your sample will retain the 37C temperature for up to 15 times longer than leaving your sample at room temperature. Then, replace the beads back into the water bath when you are done, to warm them back up to the bath temperature. Midsci can supply these beads and answer any questions you have: http://www.midsci.com/docs/opt/bath_beads.html#drybath. Don't leave your cells out in the cold!

Reference: http://www.labarmor.com/bead_trays.html

Minimize Serum Handling for Healthier Cells

Large bottles of Fetal Bovine Serum are the norm for most labs; however they can be the source of many problems as well. Multiple freeze/thaw cycles on a bottle of FBS can damage the integrity of the components in the serum. Precipitates can form. Sterility can be compromised by opening and closing FBS bottles multiple times. Money is also wasted in extra tubes and pipettes, as well as time spent aliquoting and tube labeling. Wouldn't it be nice if there was a way to start with smaller aliquots?

Introducing BioWest 50ml FBS aliquots, which come conveniently packaged in 10 bottles per case. The pre-aliquoted bottles allow everyone in the lab to have their own, to prevent any contamination, freeze/thaw problems, etc. For more information, go to this website: http://thelabshark.com/cat/prodprice2_Detail.cfm?ID=3104

Ergonomic Cell Culture Techniques

Working in the tissue culture hood all day can be a pain...literally. Ergonomic experts from all over the country, at places such as the CDC, NIH, UCLA, etc. agree that pipettor strain is a huge problem. From the repetitive motions to the posture and twisting done to complete the task, pipetting takes its toll on your body. These principles also apply to using pipet fillers in a tissue culture hood. Moving your hand and arm up and around inside the tissue culture hood, keeping the serological pipet from touching anything, and doing it for possibly hours at a time, keep the body from staying at a neutral ergonomic position.

The TPP serological pipettes help to alleviate some of the strain caused by pipetting when doing cell culture. The pipettes have a shorter design to allow for easier maneuvering around the hood. http://tpp-us.com/docs/tpp-serological-pipettes.html . Two new pipettes take the concept to a whole new level. The 25/40ml and 50/70ml pipettes include reservoirs for media at the top of the pipette to allow for a shorter design, but also allow the pipettes to hold more liquid to lessen the repetitive motion of filling tissue culture vessels http://tpp-us.com/docs/25_40ml_and_50_70ml_Reservoir_Serological_Pipettes.html . If you combine the TPP serological pipettes with the Drummond Ergonomic Pipet-Aid XL, you get a complete ergonomic pipetting system. The Pipet-Aid XL has an innovative design that has a longer handle which lowers your arm to a more neutral position. It also has an adjustable sliding hand rest and a removable stand for more convenient comfort. For more information on the Pipet-Aid XL, go to the MIDSCI website, http://thelabshark.com/cat/prodprice2_Detail.cfm?ID=2694 .

References: http://www.vistalab.com/pdf/PittConPoster1.pdf http://www.cdc.gov/od/ohs/Ergonomics/labergo.htm http://www.cdph.ca.gov/programs/hesis/Documents/labwork.pdf

Alternative to Hemocytometer Cell Counting

Using a hemocytometer to count cells is a long, arduous process. Diluting to get the optimal cell density and hand-counting cells while crouching over a microscope for long periods of time, just to count one sample, is not an optimal use of researchers' time. There is also a bias that comes with counting by hand: is this cell in or out of the grid and do I count this group of cells as one or many? Hemocytometers are very fragile and expensive if broken and to handle the device for each sample with cleanings in between increases the risk of breaking or scratching the hemocytometer. To combat this issue, TPP has designed the PCV Cell Counting System. Users simply load a sample of cell suspension into the PCV Tube, spin for 1 minute in a microcentrifuge to pellet the cells into the capillary at the bottom of the tube, and then accurately determine the cell pellet volume by eye or with the "Easy Read" measuring device. The cell pellet volume can then be utilized in conjunction with pre-determined conversion factors to quickly calculate cell number, protein production, metabolic activity, bacteria number, etc. What do you use your PCV tubes for? How do you like them?

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